By using static opaque chamber and gas chromatography, this paper studied the dynamic changes of N2O fluxes and their relationships with soil temperature, soil moisture content, and soil nitrate and ammonium contents in an intensively managed greenhouse celery-Tung choy-Bok choy-amaranth rotation field and in a bare fallow land in Nanjing suburb. The cumulative N2O emission from the rotation vegetable field was as high as 137.2 kg N x hm(-2), being significantly higher than that from the bare fallow land (29.2 kg N x hm(-2)), and the N2O-N emission factor of the rotation vegetable field ecosystem was up to 4.6%. In the rotation field, the planting of Tung choy had the greatest contribution to the annual cumulative N2O emission, occupying 53.5% of the total, followed by the planting of Bok choy (31.9%), celery field (4.5%), and amaranth (4.8%). The N2O flux of the rotation field had significant positive correlation with soil temperature, the Q10 being 2.80, but no significant correlations with soil moisture content and soil nitrate and ammonium contents.