The M5nr: a novel non-redundant database containing protein sequences and annotations from multiple sources and associated tools

BMC Bioinformatics. 2012 Jun 21:13:141. doi: 10.1186/1471-2105-13-141.


Background: Computing of sequence similarity results is becoming a limiting factor in metagenome analysis. Sequence similarity search results encoded in an open, exchangeable format have the potential to limit the needs for computational reanalysis of these data sets. A prerequisite for sharing of similarity results is a common reference.

Description: We introduce a mechanism for automatically maintaining a comprehensive, non-redundant protein database and for creating a quarterly release of this resource. In addition, we present tools for translating similarity searches into many annotation namespaces, e.g. KEGG or NCBI's GenBank.

Conclusions: The data and tools we present allow the creation of multiple result sets using a single computation, permitting computational results to be shared between groups for large sequence data sets.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Computational Biology
  • Databases, Nucleic Acid
  • Databases, Protein*
  • Metagenomics
  • Proteins / chemistry
  • Proteins / genetics
  • Software*


  • Proteins