Effects of inositol 1,4,5-trisphosphate on calcium release from the endoplasmic reticulum and Golgi apparatus in mouse mammary epithelial cells: a comparison during pregnancy and lactation

Cell Biochem Funct. 1990 Oct;8(4):191-8. doi: 10.1002/cbf.290080402.


It has been established that inositol 1,4,5-trisphosphate(IP3) is responsible for the mobilization of calcium(Ca2+) from intracellular locations in a wide variety of tissues, and that this response triggers the stimulation of several hormones and neurotransmitters. However, these phenomena have yet to be examined in the mammary epithelium. Ca2+ uptake from the medium into the endoplasmic reticulum(ER) and Golgi apparatus in vitro in both pregnant and lactating mouse mammary epithelial cells was studied and a strong Ca2+ release from these organelles into the medium with the use of IP3 was shown. The Ca2+ uptake and its release due to IP3 was also usually greater during pregnancy than lactation.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Calcimycin / pharmacology
  • Calcium / metabolism*
  • Culture Media
  • Endoplasmic Reticulum / drug effects
  • Endoplasmic Reticulum / metabolism
  • Epithelial Cells
  • Epithelium / drug effects
  • Epithelium / metabolism
  • Female
  • Golgi Apparatus / drug effects
  • Golgi Apparatus / metabolism
  • In Vitro Techniques
  • Inositol 1,4,5-Trisphosphate / pharmacology*
  • Lactation / metabolism*
  • Mammary Glands, Animal / cytology
  • Mammary Glands, Animal / drug effects*
  • Mammary Glands, Animal / metabolism
  • Mice
  • Mice, Inbred Strains
  • Pregnancy
  • Pregnancy, Animal / metabolism*
  • Subcellular Fractions / ultrastructure


  • Culture Media
  • Calcimycin
  • Inositol 1,4,5-Trisphosphate
  • Calcium