A map of nucleosome positions in yeast at base-pair resolution

Nature. 2012 Jun 28;486(7404):496-501. doi: 10.1038/nature11142.


The exact positions of nucleosomes along genomic DNA can influence many aspects of chromosome function. However, existing methods for mapping nucleosomes do not provide the necessary single-base-pair accuracy to determine these positions. Here we develop and apply a new approach for direct mapping of nucleosome centres on the basis of chemical modification of engineered histones. The resulting map locates nucleosome positions genome-wide in unprecedented detail and accuracy. It shows new aspects of the in vivo nucleosome organization that are linked to transcription factor binding, RNA polymerase pausing and the higher-order structure of the chromatin fibre.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Base Pairing*
  • Cysteine / metabolism
  • DNA-Directed RNA Polymerases / metabolism
  • Genome, Fungal / genetics
  • Histones / chemistry
  • Histones / genetics
  • Histones / metabolism
  • Hydroxyl Radical / metabolism
  • Nucleosomes / genetics*
  • Nucleosomes / metabolism
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism
  • Sequence Analysis, DNA
  • Transcription Factors / metabolism


  • Histones
  • Nucleosomes
  • Transcription Factors
  • Hydroxyl Radical
  • DNA-Directed RNA Polymerases
  • Cysteine

Associated data

  • GEO/GSE36063