Microarray analysis of the gene expression profile of HMC-1 mast cells following Schizonepeta tenuifolia Briquet treatment

Cell Immunol. May-Jun 2012;277(1-2):58-65. doi: 10.1016/j.cellimm.2012.05.012. Epub 2012 Jun 5.

Abstract

It has long been believed that mast cells play a crucial role in the development of many physiological changes during immediate allergic responses. This study was conducted to evaluate the anti-inflammation mechanism of Schizonepeta tenuifolia (ST) extract and ST purified chemicals on the PMA plus A23187-induced stimulation of HMC-1 human mast cells. ST, rosmarinic acid, pulegone, and 2α,3α,24-thrihydrooxylen-12en-28oic acid treatment of HMC-1 cells led to significant suppression of pro-inflammatory cytokines (IL-6, IL-8, and TNF-α) in a dose dependent manner. In addition, the results of the microarray and real-time RT-PCR analyses revealed that ST regulates several pathways, including the cytokine-cytokine receptor interaction (CCRI), MAPK, and the Toll-like receptor (TLR) signaling pathways. ST may be useful for the treatment of inflammation disease via anti-inflammation activity that occurs through inhibition of the CCRI, MAPK, and TLR signaling pathways.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
  • Calcimycin / pharmacology
  • Calcium Ionophores / pharmacology
  • Cell Line
  • Cinnamates / pharmacology
  • Cyclohexane Monoterpenes
  • Cytokines / biosynthesis
  • Depsides / pharmacology
  • Gene Expression Profiling
  • Humans
  • Lamiaceae*
  • Mast Cells / drug effects*
  • Mast Cells / metabolism
  • Monoterpenes / pharmacology
  • Oleanolic Acid / analogs & derivatives
  • Oleanolic Acid / pharmacology
  • Oligonucleotide Array Sequence Analysis
  • Plant Extracts / pharmacology
  • Signal Transduction / drug effects

Substances

  • 2,3,24-trihydroxyolean-12-en-28-oic acid
  • Anti-Inflammatory Agents, Non-Steroidal
  • Calcium Ionophores
  • Cinnamates
  • Cyclohexane Monoterpenes
  • Cytokines
  • Depsides
  • Monoterpenes
  • Plant Extracts
  • Calcimycin
  • pulegone
  • Oleanolic Acid
  • rosmarinic acid