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, 74 (1), 88-90

Spectrophotometry-based Detection of Carbapenemase Producers Among Enterobacteriaceae

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Spectrophotometry-based Detection of Carbapenemase Producers Among Enterobacteriaceae

Sandrine Bernabeu et al. Diagn Microbiol Infect Dis.

Abstract

Carbapenem-hydrolyzing ß-lactamases are the most powerful ß-lactamases being able to hydrolyse almost all ß-lactams. They are mostly of the KPC, VIM, IMP, NDM, and OXA-48 type. A spectrophotometry technique based on analysis of the imipenem hydrolysis has been developed that differentiated carbapenemase- from noncarbapenemase producers. This inexpensive technique adapted to screening of carbapenemase producers may be implemented in any reference laboratory worldwide.

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