Assessment of DNA damage using chromosomal aberrations assay in lymphocytes of waterpipe smokers

Int J Occup Med Environ Health. 2012 Jun;25(3):218-24. doi: 10.2478/S13382-012-0027-5. Epub 2012 Jun 22.


Objectives: The aim of this study was to investigate the genotoxicity of waterpipe smoking in the lymphocytes of waterpipe smokers using chromosomal aberrations (CAs) assay.

Materials and methods: Fifty waterpipe smokers and 18 healthy non-smokers volunteered to participate in the study. Additionally, 18 heavy cigarette smokers were recruited for comparison. Chromosomal aberrations (CAs) assay was used to evaluate DNA damage in the lymphocytes.

Results: The results showed that similarly to cigarette smoking, waterpipe smoking significantly increased the frequencies of CAs (p < 0.01). In addition, the frequencies of CAs increased with more waterpipe use.

Conclusions: Waterpipe smoking causes DNA damage to lymphocytes and the damage increases with more waterpipe use.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Chromosome Aberrations / chemically induced*
  • DNA Damage / genetics*
  • Humans
  • Jordan
  • Lymphocytes / drug effects*
  • Smoking / adverse effects*
  • Tobacco / adverse effects
  • Young Adult