miR-26b promotes granulosa cell apoptosis by targeting ATM during follicular atresia in porcine ovary

PLoS One. 2012;7(6):e38640. doi: 10.1371/journal.pone.0038640. Epub 2012 Jun 21.

Abstract

More than 99% of ovarian follicles undergo atresia in mammals, but the mechanism of follicular atresia remains to be elucidated. In this study, we explored microRNA (miRNA) regulation of follicular atresia in porcine ovary. A miRNA expression profile was constructed for healthy, early atretic, and progressively atretic follicles, and the differentially expressed miRNAs were selected and analyzed. We found that miR-26b, which was upregulated during follicular atresia, increased the number of DNA breaks and promoted granulosa cell apoptosis by targeting the ataxia telangiectasia mutated gene directly in vitro.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis*
  • Ataxia Telangiectasia Mutated Proteins
  • Cell Cycle Proteins / metabolism*
  • DNA Damage
  • DNA-Binding Proteins / metabolism*
  • Estradiol / metabolism
  • Female
  • Follicular Atresia / metabolism*
  • Follicular Fluid / metabolism
  • Gene Expression Profiling
  • Granulosa Cells / cytology*
  • Luminescence
  • MicroRNAs / biosynthesis*
  • MicroRNAs / genetics
  • Ovarian Follicle / metabolism
  • Ovary / metabolism*
  • Progesterone / metabolism
  • Protein-Serine-Threonine Kinases / metabolism*
  • Swine
  • Tissue Distribution
  • Tumor Suppressor Proteins / metabolism*
  • Up-Regulation

Substances

  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • MicroRNAs
  • Tumor Suppressor Proteins
  • Progesterone
  • Estradiol
  • Ataxia Telangiectasia Mutated Proteins
  • Protein-Serine-Threonine Kinases