Six new chalcones from Angelica keiskei inducing adiponectin production in 3T3-L1 adipocytes

Biosci Biotechnol Biochem. 2012;76(5):961-6. doi: 10.1271/bbb.110976. Epub 2012 May 7.


Angelica keiskei (Ashitaba in Japanese), a traditional herb in Japan, contains abundant prenylated chalcones. It has been reported that the chalcones from A. keiskei showed such bioactivities as anti-bacterial, anti-cancer and anti-diabetic effects. Xanthoangelol, 4-hydroxyderricin and six new chalcones were isolated in this study from an ethanol extract of A. keiskei by octadecyl silyl (ODS) and silica gel chromatography, and identified by 1D- and 2D-nuclear magnetic resonance (NMR) and high-resolution mass spectrometric analyses. The chalcones from A. keiskei markedly increased the expression of the adiponectin gene and the production of adiponectin in 3T3-L1 adipocytes. These results suggest that the chalcones from A. keiskei might be useful for preventing the metabolic syndrome.

MeSH terms

  • Adipocytes / cytology
  • Adipocytes / drug effects*
  • Adipocytes / metabolism
  • Adiponectin / biosynthesis*
  • Adiponectin / genetics
  • Angelica / chemistry*
  • Animals
  • Cell Line
  • Chalcone / analogs & derivatives*
  • Chalcone / isolation & purification
  • Chalcone / pharmacology
  • Chromatography
  • Ethanol / chemistry
  • Gene Expression / drug effects
  • Hypoglycemic Agents / isolation & purification*
  • Hypoglycemic Agents / pharmacology
  • Magnetic Resonance Spectroscopy
  • Mass Spectrometry
  • Mice
  • Plant Roots / chemistry*


  • Adiponectin
  • Hypoglycemic Agents
  • Ethanol
  • 4-hydroxyderricin
  • Chalcone
  • xanthoangelol