Non-culture detection of Streptococcus agalactiae (Lancefield group B Streptococcus) in clinical samples by real-time PCR

J Med Microbiol. 2012 Aug;61(Pt 8):1086-1090. doi: 10.1099/jmm.0.042879-0. Epub 2012 Jun 26.

Abstract

A real-time PCR assay targeting the cylB gene was developed to detect Streptococcus agalactiae [Lancefield group B Streptococcus (GBS)] from clinical samples. A total of 110 blood culture-negative samples [75 cerebrospinal fluid (CSF) and 35 EDTA blood samples] from neonates with probable GBS sepsis or meningitis were analysed. Among these, 16 of the 75 CSF samples were positive [21.3%, 95% confidence interval (CI) 12.7-32.3%] and two of the 35 EDTA blood samples were positive (5.7%, 95% CI 0.7-19.2%). The proportion testing positive in the CSF samples was significantly higher than in the EDTA blood samples (P=0.05, Fisher's exact test). Overall, this real-time PCR assay was shown to be superior to culture methods for detection of GBS from CSF and EDTA blood samples.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Bacteriological Techniques / methods*
  • Blood / microbiology
  • Cerebrospinal Fluid / microbiology
  • Humans
  • Infant, Newborn
  • Meningitis / diagnosis
  • Meningitis / microbiology
  • Molecular Diagnostic Techniques / methods*
  • Real-Time Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Sepsis / diagnosis
  • Sepsis / microbiology
  • Streptococcal Infections / diagnosis*
  • Streptococcal Infections / microbiology*
  • Streptococcus agalactiae / genetics
  • Streptococcus agalactiae / isolation & purification*