Chloroplast β-barrel proteins are assembled into the mitochondrial outer membrane in a process that depends on the TOM and TOB complexes

J Biol Chem. 2012 Aug 10;287(33):27467-79. doi: 10.1074/jbc.M112.382093. Epub 2012 Jun 28.

Abstract

Membrane-embedded β-barrel proteins are found in the outer membranes (OM) of Gram-negative bacteria, mitochondria and chloroplasts. In eukaryotic cells, precursors of these proteins are synthesized in the cytosol and have to be sorted to their corresponding organelle. Currently, the signal that ensures their specific targeting to either mitochondria or chloroplasts is ill-defined. To address this issue, we studied targeting of the chloroplast β-barrel proteins Oep37 and Oep24. We found that both proteins can be integrated in vitro into isolated plant mitochondria. Furthermore, upon their expression in yeast cells Oep37 and Oep24 were exclusively located in the mitochondrial OM. Oep37 partially complemented the growth phenotype of yeast cells lacking Porin, the general metabolite transporter of this membrane. Similarly to mitochondrial β-barrel proteins, Oep37 and Oep24 expressed in yeast cells were assembled into the mitochondrial OM in a pathway dependent on the TOM and TOB complexes. Taken together, this study demonstrates that the central mitochondrial components that mediate the import of yeast β-barrel proteins can deal with precursors of chloroplast β-barrel proteins. This implies that the mitochondrial import machinery does not recognize signals that are unique to mitochondrial β-barrel proteins. Our results further suggest that dedicated targeting factors had to evolve in plant cells to prevent mis-sorting of chloroplast β-barrel proteins to mitochondria.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / genetics
  • Arabidopsis / metabolism*
  • Biological Transport / physiology
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Chloroplast Proteins / genetics
  • Chloroplast Proteins / metabolism*
  • Gene Expression
  • Genetic Complementation Test
  • Ion Channels
  • Mitochondria / genetics
  • Mitochondria / metabolism*
  • Peas / genetics
  • Peas / metabolism*
  • Plant Proteins
  • Protein Sorting Signals / physiology*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism*

Substances

  • Carrier Proteins
  • Chloroplast Proteins
  • Ion Channels
  • OEP24 protein, Pisum sativum
  • Plant Proteins
  • Protein Sorting Signals
  • Saccharomyces cerevisiae Proteins
  • TOM translocase