Structural insights for engineering binding proteins based on non-antibody scaffolds

Curr Opin Struct Biol. 2012 Aug;22(4):413-20. doi: 10.1016/ Epub 2012 Jun 27.


Engineered binding proteins derived from non-antibody scaffolds constitute an increasingly prominent class of reagents in both research and therapeutic applications. The growing number of crystal structures of these 'alternative' scaffold-based binding proteins in complex with their targets illustrate the mechanisms of molecular recognition that are common among these systems and those unique to each. This information is useful for critically assessing and improving/expanding engineering strategies. Furthermore, the structural features of these synthetic proteins produced under tightly controlled, directed evolution deepen our understanding of the underlying principles governing molecular recognition.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Amino Acid Sequence
  • Binding Sites
  • Humans
  • Models, Molecular
  • Peptide Library
  • Protein Binding
  • Protein Engineering*
  • Protein Interaction Domains and Motifs
  • Proteins / chemistry*
  • Proteins / genetics


  • Peptide Library
  • Proteins