Functional roles of protein splicing factors

Biosci Rep. 2012 Aug;32(4):345-59. doi: 10.1042/BSR20120007.

Abstract

RNA splicing is one of the fundamental processes in gene expression in eukaryotes. Splicing of pre-mRNA is catalysed by a large ribonucleoprotein complex called the spliceosome, which consists of five small nuclear RNAs and numerous protein factors. The spliceosome is a highly dynamic structure, assembled by sequential binding and release of the small nuclear RNAs and protein factors. DExD/H-box RNA helicases are required to mediate structural changes in the spliceosome at various steps in the assembly pathway and have also been implicated in the fidelity control of the splicing reaction. Other proteins also play key roles in mediating the progression of the spliceosome pathway. In this review, we discuss the functional roles of the protein factors involved in the spliceosome pathway primarily from studies in the yeast system.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Base Sequence
  • DEAD-box RNA Helicases / physiology
  • Humans
  • Models, Molecular
  • Molecular Sequence Data
  • RNA Splicing*
  • RNA, Messenger / metabolism*
  • Ribonucleoproteins, Small Nuclear / physiology
  • Spliceosomes / metabolism
  • Spliceosomes / physiology*

Substances

  • RNA, Messenger
  • Ribonucleoproteins, Small Nuclear
  • DEAD-box RNA Helicases