Purified protein derivatives of tuberculin--past, present, and future

FEMS Immunol Med Microbiol. 2012 Dec;66(3):273-80. doi: 10.1111/j.1574-695X.2012.01002.x. Epub 2012 Aug 1.

Abstract

The tuberculin skin test, which involves monitoring the immune reaction to an injection of purified protein derivative (PPD), has been the most widely used method for detecting infection with Mycobacterium tuberculosis since its development in 1930s. Until recently, the molecular composition of PPD was unknown. This thwarted the discovery of improved skin testing reagents and drastically hindered efforts to define the mechanism of action. Proteomic evaluation of PPD combined with a detailed analysis in the guinea pig model of tuberculosis led to further definition of the molecular composition of PPD. This communication reviews the history and current status of PPD, in addition to describing candidate next-generation PPD reagents, based on the use of an individual protein or protein cocktails.

Publication types

  • Historical Article
  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Animals
  • Guinea Pigs
  • History, 20th Century
  • History, 21st Century
  • Humans
  • Tuberculin Test / history
  • Tuberculin Test / methods*
  • Tuberculin Test / trends
  • Tuberculin* / chemistry
  • Tuberculin* / history
  • Tuberculosis / diagnosis*
  • Tuberculosis / immunology*
  • Tuberculosis / prevention & control
  • Tuberculosis Vaccines / immunology*

Substances

  • Tuberculin
  • Tuberculosis Vaccines