Effect of in vitro chondrogenic differentiation of autologous mesenchymal stem cells on cartilage and subchondral cancellous bone repair in osteoarthritis of temporomandibular joint

Int J Oral Maxillofac Surg. 2013 Feb;42(2):240-8. doi: 10.1016/j.ijom.2012.05.030. Epub 2012 Jul 2.


This study investigated the effects of in vitro chondrogenic differentiated mesenchymal stem cells (MSCs) on cartilage and subchondral cancellous bone in temporomandibular joint osteoarthritis (TMJOA). Four weeks after induction of osteoarthritis (OA), the joints received hylartin solution, non-chondrogenic MSCs or in vitro chondrogenic differentiated MSCs. The changes in cartilage and subchondral cancellous bone were evaluated by histology, reverse transcription polymerase chain reaction and micro-computed tomography (CT). Implanted cells were tracked using Adeno-LacZ labelling. The differentiated MSC-treated group had better histology than the MSC-treated group at 4 and 12 weeks, but no difference at 24 weeks. Increased mRNA expression of collegan II, aggeran, Sox9 and decreased matrix metalloproteinase 13 (MMP13) were observed in differentiated MSC-treated groups compared to the undifferentiated MSC-treated group at 4 weeks. The differentiated MSC-treated group had decreased bone volume fraction, trabecular thickness and bone surface density, and increased trabecular spacing in the subchondral cancellous bone than the undifferentiated MSC-treated group. Transplanted cells were observed at cartilage, subchondral bone, and the synovial membrane lining at 4 weeks. Intra-articular injection of MSCs could delay the progression of TMJOA, and in vitro chondrogenic induction of MSCs could enhance the therapeutic effects. This provides new insights into the role of MSCs in cell-based therapies for TMJOA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aggrecans / biosynthesis
  • Aggrecans / genetics
  • Animals
  • Bone Density
  • Bone Regeneration*
  • Cartilage, Articular
  • Cell Differentiation
  • Cells, Cultured
  • Chondrocytes / cytology
  • Chondrogenesis*
  • Collagen Type II / biosynthesis
  • Collagen Type II / genetics
  • Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) / biosynthesis
  • Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) / genetics
  • Injections, Intra-Articular
  • Matrix Metalloproteinase 13 / biosynthesis
  • Matrix Metalloproteinase 13 / genetics
  • Mesenchymal Stem Cell Transplantation / methods*
  • Mesenchymal Stem Cells / cytology*
  • Osteoarthritis / diagnostic imaging
  • Osteoarthritis / therapy*
  • Rabbits
  • Real-Time Polymerase Chain Reaction
  • SOX9 Transcription Factor / biosynthesis
  • SOX9 Transcription Factor / genetics
  • Temporomandibular Joint Disorders / diagnostic imaging
  • Temporomandibular Joint Disorders / therapy*
  • X-Ray Microtomography


  • Aggrecans
  • Collagen Type II
  • SOX9 Transcription Factor
  • Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+)
  • Matrix Metalloproteinase 13