The correlation between hepatitis B virus infection and IL-27

Biomed Mater Eng. 2012;22(1-3):187-93. doi: 10.3233/BME-2012-0706.

Abstract

Objective: To investigate the correlation between HBV infection and interleukin 27 (IL-27).

Methods: Serum IL-27 levels in patients with HBV infection and in healthy individuals were measured by ELISA, regulation of IL-27 by HBV in vitro were analyzed by RT-PCR and ELISA.

Results: IL-27 was significantly elevated in patients as compared to healthy individuals (P<0.001). IL-27 was also detected at higher levels in patients with liver cirrhosis or hepatocellular carcinoma than those with acute hepatitis B or chronic hepatitis B (P<0.05). We also found that IL-27 expression was influenced by HBV e antigen. In addition, our in vitro studies demonstrated that mRNA and protein expression were all stimulated in cells transfected with infectious HBV clone.

Conclusions: IL-27 would be a novel cytokine for clinical management of HBV-infected patients, which may establish novel HBV therapeutic approaches.

MeSH terms

  • Adult
  • Aged
  • Cell Line, Tumor
  • Female
  • Gene Expression Regulation
  • Hepatitis B / blood
  • Hepatitis B / genetics
  • Hepatitis B / immunology*
  • Hepatitis B e Antigens / immunology
  • Hepatitis B virus / immunology*
  • Humans
  • Interleukin-17 / blood
  • Interleukin-17 / genetics
  • Interleukin-17 / immunology*
  • Male
  • Middle Aged

Substances

  • Hepatitis B e Antigens
  • Interleukin-17