Epigenetic alterations in human pluripotent stem cells: a tale of two cultures

Cell Stem Cell. 2012 Jul 6;11(1):9-15. doi: 10.1016/j.stem.2012.06.012.


Human somatic cells can be reprogrammed into induced pluripotent stem cells (hiPSCs) with wide lineage differentiation potential in culture. However, reprogramming and long-term culture can also induce abnormalities in these pluripotent cells. This minireview discusses recent studies that have identified changes in imprinted gene expression and erosion of X chromosome inactivation in female hiPSCs and how understanding the sources and consequences of epigenetic variability in hiPSCs will impact disease modeling and clinical application in the future.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Cell Culture Techniques*
  • Cellular Reprogramming / genetics
  • Epigenesis, Genetic*
  • Humans
  • Induced Pluripotent Stem Cells / metabolism
  • Pluripotent Stem Cells / metabolism*
  • X Chromosome Inactivation