Polypodium leucotomos decreases UV-induced epidermal cell proliferation and enhances p53 expression and plasma antioxidant capacity in hairless mice

Exp Dermatol. 2012 Aug;21(8):638-40. doi: 10.1111/j.1600-0625.2012.01544.x.

Abstract

A single dose of ultraviolet radiation (UVR) induces significant changes in blood and skin of hairless mice. Oral administration of a hydrophilic extract of the fern Polypodium leucotomos (PL, 300 mg/kg during 5 days before UVR and for two additional days after irradiation) modulates some of the effects of UVR. Most significantly, PL administration reduced the number of proliferating cells by 13%, increased the number of p53(+) cells by 63%, enhanced the antioxidant plasma capacity (ORAC) by 30% and reinforced the network of dermal elastic fibres. Western blot analysis of skin antioxidant-related enzymes failed to demonstrate significant changes caused by PL. Thus, the beneficial effect of PL likely owes to its antioxidant and anti-ROS properties rather than its modulation of the expression of endogenous antioxidant systems. These data provide mechanistic clues for its efficacy as a systemic photoprotective agent with antioxidant and anti-photo-ageing properties.

Publication types

  • Letter

MeSH terms

  • Administration, Oral
  • Animals
  • Antioxidants / metabolism*
  • Cell Proliferation / drug effects*
  • Dose-Response Relationship, Radiation
  • Elastic Tissue / metabolism
  • Epidermis / drug effects
  • Epidermis / metabolism*
  • Epidermis / pathology*
  • Female
  • Mice
  • Mice, Hairless
  • Models, Animal
  • Plant Extracts / administration & dosage
  • Plant Extracts / pharmacology*
  • Polypodium*
  • Skin Aging / drug effects
  • Superoxide Dismutase / metabolism
  • Tumor Suppressor Protein p53 / metabolism*
  • Ultraviolet Rays / adverse effects*

Substances

  • Antioxidants
  • Plant Extracts
  • Tumor Suppressor Protein p53
  • Superoxide Dismutase