Identification of HLA-A24-restricted novel T Cell epitope peptides derived from P-cadherin and kinesin family member 20A

J Biomed Biotechnol. 2012;2012:848042. doi: 10.1155/2012/848042. Epub 2012 Jun 19.

Abstract

We here identified human leukocyte antigen-(HLA-)A(∗)2402-restricted epitope peptides from Cadherin 3, type 1, P-cadherin (CDH3) and kinesin family member 20A (KIF20A) that were found to be specifically expressed in cancer cells through genome-wide expression profile analysis. CDH3-10-807 peptide and KIF20A-10-66 peptide successfully induced specific CTL clones, and these selectively responded to COS7 cells expressing both HLA-A(∗)2402 and respective protein while did not respond to parental cells or COS7 cells expressing either HLA-A(∗)2402 or respective protein. Furthermore, CTL clones responded to cancer cells that endogenously express HLA-A(∗)2402 and respective protein, suggesting that CDH3-10-807 peptide and KIF20A-10-66 peptide are naturally presented on HLA-A(∗)2402 molecule of human cancer cells. Our results demonstrated that CDH3-10-807 peptide and KIF20A-10-66 peptide are novel HLA-A24-restricted tumor-associated antigens and would be applicable for CTL-inducing cancer therapies.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • COS Cells
  • Cadherins / chemistry
  • Cadherins / immunology*
  • Cell Line
  • Cell Survival / immunology
  • Chlorocebus aethiops
  • Epitopes, T-Lymphocyte / chemistry
  • Epitopes, T-Lymphocyte / immunology*
  • HLA-A24 Antigen / chemistry
  • HLA-A24 Antigen / immunology*
  • Humans
  • Interferon-gamma / immunology
  • Kinesin / chemistry
  • Kinesin / immunology*
  • Molecular Sequence Data
  • Peptides / chemistry
  • Peptides / immunology*
  • T-Lymphocytes, Cytotoxic / immunology

Substances

  • CDH3 protein, human
  • Cadherins
  • Epitopes, T-Lymphocyte
  • HLA-A24 Antigen
  • KIF20A protein, human
  • Peptides
  • Interferon-gamma
  • Kinesin