Guidelines for high-resolution genotyping of Chlamydia trachomatis using multilocus sequence analysis

Methods Mol Biol. 2012:903:51-64. doi: 10.1007/978-1-61779-937-2_4.


Chlamydia is one of the most common sexually transmitted infections worldwide and can cause ectopic pregnancies and infertility. It is therefore important to have adequate genotyping tools for investigating the spread of Chlamydia trachomatis among the population. Here, we describe a high-resolution multilocus sequence typing (MLST) system able to differentiate closely related clinical strains, which makes it ideal for short-term epidemiology and outbreak investigations. It is based on five highly variable but stable target regions which are PCR amplified and DNA sequenced.

Publication types

  • Guideline

MeSH terms

  • Chemical Precipitation
  • Chlamydia trachomatis / genetics*
  • Chlamydia trachomatis / isolation & purification*
  • DNA, Bacterial / genetics
  • DNA, Bacterial / isolation & purification
  • Electrophoresis, Agar Gel
  • Ethanol / chemistry
  • Genotyping Techniques / methods*
  • Molecular Diagnostic Techniques
  • Multilocus Sequence Typing / methods*
  • Polymerase Chain Reaction


  • DNA, Bacterial
  • Ethanol