Hydrogen sulfide and resolution of acute inflammation: A comparative study utilizing a novel fluorescent probe

Sci Rep. 2012;2:499. doi: 10.1038/srep00499. Epub 2012 Jul 9.

Abstract

Hydrogen sulfide is an essential gasotransmitter associated with numerous pathologies. We assert that hydrogen sulfide plays an important role in regulating macrophage function in response to subsequent inflammatory stimuli, promoting clearance of leukocyte infiltrate and reducing TNF-α levels in vivo following zymosan-challenge. We describe two distinct methods of measuring leukocyte hydrogen sulfide synthesis; methylene blue formation following zinc acetate capture and a novel fluorescent sulfidefluor probe. Comparison of these methods, using pharmacological tools, revealed they were complimentary in vitro and in vivo. We demonstrate the application of sulfidefluor probe to spectrofluorimetry, flow cytometry and whole animal imaging, to monitor the regulation of hydrogen sulfide synthesis in vivo during dynamic inflammatory processes. Both methodologies revealed that granulocyte infiltration negatively affects hydrogen sulfide synthesis. Our report offers an insight into the profile of hydrogen sulfide synthesis during inflammation and highlight opportunities raised by the development of novel fluorescent hydrogen sulfide probes.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chemotaxis / drug effects
  • Chemotaxis / immunology
  • Cystathionine beta-Synthase / metabolism
  • Fluorescent Dyes* / metabolism
  • Humans
  • Hydrogen Sulfide / metabolism*
  • Inflammation / diagnosis
  • Inflammation / immunology*
  • Inflammation / metabolism*
  • Leukocytes, Mononuclear / drug effects
  • Leukocytes, Mononuclear / immunology
  • Leukocytes, Mononuclear / metabolism
  • Lipopolysaccharides / immunology
  • Macrophage Activation / drug effects
  • Macrophage Activation / immunology
  • Macrophages / drug effects
  • Macrophages / immunology
  • Macrophages / metabolism
  • Male
  • Mice
  • Molecular Imaging
  • Neutrophil Infiltration / drug effects
  • Neutrophil Infiltration / immunology
  • Neutrophils / drug effects
  • Neutrophils / immunology
  • Neutrophils / metabolism
  • Peritonitis / chemically induced
  • Peritonitis / immunology
  • Peritonitis / metabolism
  • Phagocytosis / drug effects
  • Phagocytosis / immunology
  • Sulfides / administration & dosage
  • Sulfides / pharmacology

Substances

  • Fluorescent Dyes
  • Lipopolysaccharides
  • Sulfides
  • Cystathionine beta-Synthase
  • sodium bisulfide
  • Hydrogen Sulfide