Comparison of 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels using mass spectrometer and urine albumin creatinine ratio as a predictor of development of diabetic nephropathy

Free Radic Res. 2012 Oct;46(10):1291-5. doi: 10.3109/10715762.2012.710902. Epub 2012 Aug 8.

Abstract

Background: Measurement of urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) has recently become more popular as a means of assessing oxidative stress in the human body. The aim of this study is to compare the levels of urine 8-OHdG in patients with type 2 diabetes with and without nephropathy and to evaluate its role as a biochemical marker for distinguishing these patients from healthy and patients without complications.

Methods: For this purpose, 52 patients with type 2 diabetes mellitus (32 with nephropathy (DMN), 20 without nephropathy (DM)) and 20 healthy control subjects (C) were included in this study. The urine concentrations of 8-OHdG were measured by modified LC-MS/MS method and compared with the first morning voiding urine albumin/creatinine ratio (UACR) and HbA1c values of the same patients.

Results: The concentrations of urine 8-OHdG in DMN and DM patients were higher than those of the control subjects (3.47 ± 0.94, 2.92 ± 1.73, 2.1 ± 0.93 nmol/mol creatinine, respectively). But there was no statistical difference between DMN and DM (p = 0.115). There is significant correlation between urinary 8-OHdG and UACR (r = 0.501, p < 0.001). According to ROC analysis, the AUC value of HbA1c was higher than the value of the AUC of 8-OHdG (0.882 and 0.771, respectively).

Conclusions: This study shows that the urine 8-OHdG levels increase in diabetic patients. However, urinary 8-OHdG is not a useful clinical marker, compared with UACR, to predict the development of diabetic nephropathy in diabetic patients.

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Albuminuria / urine*
  • Biomarkers / urine
  • Case-Control Studies
  • Creatinine / urine*
  • Deoxyguanosine / analogs & derivatives*
  • Deoxyguanosine / urine
  • Diabetes Mellitus, Type 2 / blood
  • Diabetes Mellitus, Type 2 / urine*
  • Diabetic Nephropathies / urine*
  • Female
  • Glycated Hemoglobin A / metabolism
  • Humans
  • Male
  • Mass Spectrometry
  • Middle Aged
  • Reactive Oxygen Species
  • Tandem Mass Spectrometry / methods

Substances

  • Biomarkers
  • Glycated Hemoglobin A
  • Reactive Oxygen Species
  • hemoglobin A1c protein, human
  • 8-Hydroxy-2'-Deoxyguanosine
  • Creatinine
  • Deoxyguanosine