Molecular cloning of a cDNA encoding the human interleukin 4 receptor

Int Immunol. 1990;2(7):669-75. doi: 10.1093/intimm/2.7.669.


Using the mouse interleukin 4 (IL-4) receptor cDNA as a probe, we isolated a cDNA encoding the human IL-4 receptor (hIL-4 receptor) from a multifactor-responsive human myeloid cell line, TF1. The cDNA encodes for an open reading frame of 825 amino acids including a signal sequence (25 amino acids), the external domain (207 amino acids), a transmembrane domain (24 amino acids), and a large cytoplasmic domain (569 amino acids). The human IL-4 receptor has a 65% identity with the mouse IL-4 receptor at the nucleic acid level and retains the typical structural motif of the previously described cytokine receptor family. COS7 cells transfected with the full-length cDNA expressed high levels (140,000 sites/cell) of IL-4 binding sites, with a Kd = 80 pM, an affinity identical to that of the original TF1 cells. Similar to IL-4 responsive cells, cross-linking of [125I]IL-4 to COS7 cells transfected with the cDNA showed a major protein of 130-150 kd and minor species of 55-85 kd.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA / genetics*
  • DNA Probes
  • Humans
  • Interleukin-4 / metabolism*
  • Mice
  • Molecular Sequence Data
  • Molecular Weight
  • Receptors, Interleukin-4
  • Receptors, Mitogen / chemistry
  • Receptors, Mitogen / genetics*
  • Receptors, Mitogen / metabolism
  • Sequence Homology, Nucleic Acid
  • Species Specificity


  • DNA Probes
  • Receptors, Interleukin-4
  • Receptors, Mitogen
  • Interleukin-4
  • DNA