Important role of β1-integrin in fucoidan-induced apoptosis via caspase-8 activation

Biosci Biotechnol Biochem. 2012;76(6):1163-8. doi: 10.1271/bbb.111001. Epub 2012 Jun 7.


Fucoidan induces apoptosis by activating caspase-8 in human MCF-7 breast cancer cells, but the detailed mechanism for this is not understood. We demonstrate here that fucoidan interacted with the cell surface, and silencing the β1-integrin gene expression inhibited fucoidan-induced apoptosis accompanied by caspase-8 activation. Fucoidan induced formation of the β1-integrin-caspase-8 complex. These data indicate that β1-integrin is an important factor for the cell-surface binding of fucoidan and plays an important role in fucoidan-induced apoptosis. Fucoidan also induced recruitment of caspase-8 to the β1-integrin intracellular domain, cleaved it into the activated protein by direct combination with β1-integrin, and induced apoptosis via the caspase cascade in MCF-7 cells.

MeSH terms

  • Antineoplastic Agents / metabolism
  • Antineoplastic Agents / pharmacology*
  • Apoptosis / drug effects
  • Caspase 8 / genetics
  • Caspase 8 / metabolism*
  • Cell Line, Tumor
  • Enzyme Activation / drug effects
  • Female
  • Gene Expression
  • Gene Silencing / drug effects
  • Humans
  • Immunoprecipitation
  • Integrin beta1 / genetics
  • Integrin beta1 / metabolism*
  • Polysaccharides / metabolism
  • Polysaccharides / pharmacology*
  • Protein Binding
  • Protein Structure, Tertiary
  • RNA, Small Interfering / genetics
  • Surface Plasmon Resonance


  • Antineoplastic Agents
  • Integrin beta1
  • Polysaccharides
  • RNA, Small Interfering
  • fucoidan
  • Caspase 8