Effects of hydrogen-rich saline treatment on polymicrobial sepsis

J Surg Res. 2013 May;181(2):279-86. doi: 10.1016/j.jss.2012.06.058. Epub 2012 Jul 7.


Background: Hydrogen has been reported to selectively reduce hydroxyl radicals and peroxynitrite anion in many pathologic processes. This study aimed to test the hypothesis that hydrogen-rich saline (HRS) may ameliorate organ dysfunction in a rat model of polymicrobial sepsis.

Methods: Sepsis was induced in male Sprague-Dawley rats by cecal ligation and puncture (CLP). Twenty-four rats were equally assigned to Sham group, CLP group, and CLP + HRS group (n = 8). At 0, 6, and 18 h after CLP or sham operation, rats received an intraperitoneal injection of HRS (5 mL/kg) or the same volume of normal saline. Malondialdehyde, superoxide dismutase activities, inflammatory mediators, pulmonary nitric oxide, myeloperoxidase activities, wet-to-dry weight ratio, histologic scores, apoptotic analysis, alanine aminotransferase, creatinine, and blood urea nitrogen were assessed at 24 h after operation. The 7-d survival rate was also recorded.

Results: HRS administration significantly reduced the serum high-mobility group box, alanine aminotransferase, creatinine, and blood urea nitrogen levels; the pulmonary interleukin 6, high-mobility group box, nitric oxide, and malondialdehyde levels; and the wet-to-dry weight ratio, total histologic scores, and terminal deoxynucleotidyl transferase dUTP nick end labeling-positive cells, whereas it increased the superoxide dismutase activities 24 h after CLP when compared with the CLP group. However, there was no significant difference in survival rate between the CLP + HRS and CLP groups.

Conclusions: HRS has potential protective effects against sepsis by decreasing proinflammatory responses, oxidative stress, and apoptosis in a rat model of polymicrobial sepsis.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / therapeutic use*
  • Apoptosis / drug effects
  • Biomarkers / metabolism
  • Cecum / surgery
  • Coinfection / drug therapy*
  • Coinfection / etiology
  • Coinfection / metabolism
  • Coinfection / mortality
  • Hydrogen / therapeutic use*
  • Inflammation Mediators / metabolism
  • Injections, Intraperitoneal
  • Kaplan-Meier Estimate
  • Male
  • Multiple Organ Failure / etiology
  • Multiple Organ Failure / prevention & control*
  • Oxidative Stress / drug effects
  • Pharmaceutical Vehicles
  • Random Allocation
  • Rats
  • Sepsis / drug therapy*
  • Sepsis / etiology
  • Sepsis / metabolism
  • Sepsis / mortality
  • Sodium Chloride
  • Treatment Outcome


  • Antioxidants
  • Biomarkers
  • Inflammation Mediators
  • Pharmaceutical Vehicles
  • Sodium Chloride
  • Hydrogen