Periodate or glutaraldehyde for preparing peroxidase conjugates?

J Immunol Methods. 1979;30(3):245-55. doi: 10.1016/0022-1759(79)90098-x.


Conjugates of horseradish peroxidase with antibodies (anti-human IgG (H + L)) or their Fab' fragments were prepared according to the newest modification of the periodate (P-) method or the two-step glutaraldehyde (G-) method. The conjugates were analysed by gel chromatography and subsequently tested in three different applications. For tissue immunohistochemistry on sections of lupus erythematosus skin, G-conjugates were preferred to polymeric P-conjugates. In ELISA for detection of human antibodies against penicillin P-conjugates were superior to G-conjugates. For the detection of surface Ig on lymphoid cells both types of conjugate were more or less equally suitable. A scheme for the most suitable combinations of method of preparation and field of application is given.

Publication types

  • Comparative Study

MeSH terms

  • Aldehydes / pharmacology*
  • Animals
  • Antibodies
  • Antigens, Surface
  • Enzyme-Linked Immunosorbent Assay
  • Fluorescent Antibody Technique
  • Glutaral / pharmacology*
  • Histocytochemistry
  • Horseradish Peroxidase
  • Humans
  • Immunoglobulin Fab Fragments
  • Periodic Acid / pharmacology*
  • Rabbits


  • Aldehydes
  • Antibodies
  • Antigens, Surface
  • Immunoglobulin Fab Fragments
  • Periodic Acid
  • Horseradish Peroxidase
  • Glutaral