MicroRNA-145 suppresses mouse granulosa cell proliferation by targeting activin receptor IB

FEBS Lett. 2012 Sep 21;586(19):3263-70. doi: 10.1016/j.febslet.2012.06.048. Epub 2012 Jul 13.

Abstract

MicroRNAs (miRNAs) are a class of 21- to 25-nucleotide non-coding RNAs, some of which are important gene regulators involved in folliculogenesis. In this study, we used CCK-8, real-time PCR and Western blot assays to demonstrate that miR-145 inhibits mouse granulosa cell (mGC) proliferation. Combined with the results of luciferase reporter assays that studied the 3'-untranslated region of ACVRIB mRNA, these assays identified ACVRIB as a direct target of miR-145. The ectopic expression of miR-145 reduced the levels of both ACVRIB mRNA and protein and also interfered with activin-induced Smad2 phosphorylation. Altogether, this study revealed that miR-145 suppresses mGC proliferation by targeting ACVRIB.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions
  • Activin Receptors, Type I / genetics*
  • Activin Receptors, Type I / metabolism*
  • Activins / metabolism
  • Activins / pharmacology
  • Animals
  • Base Sequence
  • Cell Proliferation / drug effects
  • Cells, Cultured
  • Cyclin D2 / genetics
  • Cyclin D2 / metabolism
  • Female
  • Granulosa Cells / cytology*
  • Granulosa Cells / drug effects
  • Granulosa Cells / metabolism*
  • HEK293 Cells
  • Humans
  • Mice
  • Mice, Inbred ICR
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism*
  • Phosphorylation
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Signal Transduction
  • Smad2 Protein / metabolism

Substances

  • 3' Untranslated Regions
  • Ccnd2 protein, mouse
  • Cyclin D2
  • MIRN145 microRNA, mouse
  • MicroRNAs
  • RNA, Messenger
  • Smad2 Protein
  • Smad2 protein, mouse
  • activin A
  • Activins
  • Activin Receptors, Type I
  • Acvr1b protein, mouse