An excitation ratiometric Zn2+ sensor with mitochondria-targetability for monitoring of mitochondrial Zn2+ release upon different stimulations

Chem Commun (Camb). 2012 Aug 28;48(67):8365-7. doi: 10.1039/c2cc33648k. Epub 2012 Jul 13.

Abstract

A mitochondria-targeted fluorescent sensor (Mito-ST), constructed by integrating a sulfamoylbenzoxadiazole fluorophore with a phosphonium group, displays the specific Zn(2+)-induced hypsochromic shifts of both excitation (69 nm) and emission (35 nm) maxima. Its ratiometric Zn(2+) imaging ability via dual excitation mode has been applied in MCF-7 cells to clarify the different behaviours of mitochondrial Zn(2+) release stimulated by H(2)O(2) and SNOC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Fluorescent Dyes / chemistry*
  • Humans
  • Hydrogen Peroxide / chemistry
  • MCF-7 Cells
  • Mitochondria / chemistry*
  • Mitochondria / metabolism*
  • Molecular Structure
  • Optical Imaging
  • Zinc / chemistry*
  • Zinc / pharmacokinetics

Substances

  • Fluorescent Dyes
  • Hydrogen Peroxide
  • Zinc