Screening for glycosidase activities of lactic acid bacteria as a biotechnological tool in oenology

World J Microbiol Biotechnol. 2012 Apr;28(4):1423-32. doi: 10.1007/s11274-011-0942-9. Epub 2011 Nov 15.


The aim of this study was to evaluate the ability from a number of lactic acid bacteria isolated from different sources to produce glycosidase enzymes. Representative isolates (225) from clusters obtained after genotyping, using randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) analysis, of 1,464 isolates, were screened for β-D-glucosidase activity. Thirty-five of them were selected for subsequent analysis. These strains were able to hydrolyze α-D-glucopyranoside, β-D-xylopyranoside and α-L-arabinofuranoside although β-D-glucosidase activity was the predominant activity for 22 of the selected strains. Only some of them did so with α-L-rhamnopyranoside. All of these were from wine samples and were identified as belonging to the Oenococcus oeni species using Amplification and Restriction Analysis of 16S-rRNA gene (16S-ARDRA). When the influence of pH, temperature and ethanol or sugars content on β-D-glucosidase activity was assayed, a strain-dependent response was observed. The β-D-glucosidase activity occurred in both whole and sonicated cells but not in the supernatants from cultures or obtained after cell sonication. Strains 10, 17, 21, and 23 retained the most β-D-glucosidase activity when they were assayed at the conditions of temperature, pH, ethanol and sugar content used in winemaking. These results suggest that these strains could be used as a source of glycosidase enzymes for use in winemaking.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biotechnology / methods*
  • DNA, Bacterial / genetics
  • Enzyme Inhibitors / metabolism
  • Ethanol / metabolism
  • Genes, rRNA
  • Glycoside Hydrolases / analysis*
  • Hydrogen-Ion Concentration
  • Lactobacillales / classification
  • Lactobacillales / enzymology*
  • Lactobacillales / genetics
  • Lactobacillales / isolation & purification
  • Mass Screening / methods*
  • Molecular Typing
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • RNA, Ribosomal, 16S / genetics
  • Temperature
  • Wine / microbiology*


  • DNA, Bacterial
  • Enzyme Inhibitors
  • RNA, Ribosomal, 16S
  • Ethanol
  • Glycoside Hydrolases