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. 2012 Jul 19;13(8):705-6.
doi: 10.1038/ni.2347.

Lower TCR repertoire diversity in Traj18-deficient mice

Romain Bedel, Jennifer L Matsuda, Manfred Brigl, Janice White, John Kappler, Philippa Marrack, Laurent Gapin

Lower TCR repertoire diversity in Traj18-deficient mice

Romain Bedel et al. Nat Immunol. .
No abstract available

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Conflict of interest statement

Competing Financial Interests:

The authors declare no competing financial interests.

Figures

Fig 1
Fig 1
Impaired TCRα diversity in TRAJ18−/− mice. The frequency of TRAJ gene usage for productive, in-frame, rearrangements involving the TRAV11, TRAV9 and TRAV12 family gene segments in sorted CD69− double positive (CD4+CD8+) thymocytes from C57BL/6, CD1d1d2−/− and TRAJ18−/− mice is shown. Rearrangements for each V gene family were amplified by PCR with specific V primers and a specific reverse C primer. PCR products were subjected to high throughput sequencing using the 454 platform. Sequences were analyzed using an in-house software and identity of each TRAV and TRAJ genes was assigned based on sequence alignment to published sequences (www.imgt.org). The number of analyzed sequences for each sample is indicated in parenthesis. The black and red bars indicate replicate experiments from independent samples. TRAJ18−/− that have been maintained in the animal facility at National Jewish Health (NJH), were compared to the same mice that had been maintained at the Harvard Medical school mouse facility. All mice were on the C57BL/6 background and were housed in specific pathogen-free conditions. All animal studies were approved by NJH Animal Care and Use Committee. TRAJ genes are ordered from 5′ to 3′, similar to their position in the TRAJ locus.
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