Anxiolytic effect of homeopathic preparation of Pulsatilla nigricans in Swiss albino mice

Homeopathy. 2012 Jul;101(3):171-4. doi: 10.1016/j.homp.2012.05.003.


Background: The homeopathic preparation of Pulsatilla nigricans is used in the treatment of anxiety related disorders. Though in clinical use for many years, the anxiolytic activity of Pulsatilla nigricans (Puls) has not been evaluated experimentally. Hence the present study was conducted in Swiss albino mice to evaluate the anxiolytic activity of Puls and compare its activity with the standard anxiolytic drug, diazepam.

Methods: Twenty four mice were divided into 4 groups of 6 animals each, control, standard and two test groups. The control group was treated Ethyl alcohol 10 ml/kg. The standard group received Diazepam, 1 mg/kg. Puls 3x and 6x solutions were given at the dose of 10 ml/kg to the test groups. All animals were given the test and control treatments orally for 15 days. The anxiolytic effect was tested on days 1, 8 and 15 using the Elevated Plus Maze (EPM) and Open Field Test (OFT).

Results: Both diazepam and Puls showed significant anxiolytic activity in EPM and OFT test compared to control. The total number of entries and time spent in open arm in EPM was increased by both diazepam and Puls, the effect of 3x dilution of Puls was greater than diazepam. In the OFT the number of squares crossed, rearing and assisted rearings decreased with both diazepam and Puls compared to control and the anxiolytic effect of diazepam was greater than Puls. The anxiolytic effect is greater for the 3x dilution than 6x dilution of Puls.

Conclusion: The study showed an anxiolytic effect of homeopathic preparation of Pulsatilla nigricans comparable to that found with a standard drug.

MeSH terms

  • Animals
  • Anti-Anxiety Agents / pharmacology*
  • Diazepam / pharmacology
  • Homeopathy*
  • Maze Learning / drug effects
  • Mice
  • Pulsatilla*


  • Anti-Anxiety Agents
  • Diazepam