Sox2+ stem cells contribute to all epithelial lineages of the tooth via Sfrp5+ progenitors

Dev Cell. 2012 Aug 14;23(2):317-28. doi: 10.1016/j.devcel.2012.05.012. Epub 2012 Jul 19.

Abstract

The continuously growing mouse incisor serves as a valuable model to study stem cell regulation during organ renewal. Epithelial stem cells are localized in the proximal end of the incisor in the labial cervical loop. Here, we show that the transcription factor Sox2 is a specific marker for these stem cells. Sox2+ cells became restricted to the labial cervical loop during tooth morphogenesis, and they contributed to the renewal of enamel-producing ameloblasts as well as all other epithelial cell lineages of the tooth. The early progeny of Sox2-positive stem cells transiently expressed the Wnt inhibitor Sfrp5. Sox2 expression was regulated by the tooth initiation marker FGF8 and specific miRNAs, suggesting a fine-tuning to maintain homeostasis of the dental epithelium. The identification of Sox2 as a marker for the dental epithelial stem cells will facilitate further studies on their lineage segregation and differentiation during tooth renewal.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomarkers / analysis
  • Biomarkers / metabolism
  • Cell Differentiation
  • Cell Lineage*
  • Cells, Cultured
  • Epithelial Cells / chemistry
  • Epithelial Cells / cytology
  • Epithelial Cells / metabolism*
  • Fibroblast Growth Factor 8 / genetics
  • Gene Expression Regulation, Developmental
  • Intercellular Signaling Peptides and Proteins / metabolism*
  • Mice
  • MicroRNAs / genetics
  • Organ Culture Techniques
  • SOXB1 Transcription Factors / analysis
  • SOXB1 Transcription Factors / biosynthesis*
  • Stem Cells / chemistry
  • Stem Cells / cytology
  • Stem Cells / metabolism*
  • Tooth / cytology
  • Tooth / embryology
  • Tooth / growth & development
  • Tooth / metabolism*

Substances

  • Biomarkers
  • Fgf8 protein, mouse
  • Intercellular Signaling Peptides and Proteins
  • MicroRNAs
  • SOXB1 Transcription Factors
  • Sfrp5 protein, mouse
  • Sox2 protein, mouse
  • Fibroblast Growth Factor 8