Electrochemical immunosensor for label free epidermal growth factor receptor (EGFR) detection

Biosens Bioelectron. 2013 Jan 15;39(1):300-5. doi: 10.1016/j.bios.2012.06.012. Epub 2012 Jun 28.


This paper presents an electrochemical immune sensor for label free detection of epidermal growth factor receptor (EGFR) by immobilizing anti-EGFR antibody (Anti-EGFRab) on dithiobissuccinimidyl propionate (DTSP) self-assembled monolayer (SAM) on gold (Au) electrode. Electrochemical studies show that increased surface concentration of redox moieties onto Anti-EGFRab/DTSP immuno-electrode leads to high electron transport and improved sensing performance. The antigen-antibody complex demonstrates a high association constant (5×10(12)L/mol) that results in high affinity of Anti-EGFRab to EGFR, confirming that the DTSP-SAM provides a conducive environment for anti-EGFR immobilization. The electrochemical response of EA/Anti-EGFRab/DTSP/Au electrode as a function of EGFR concentrations exhibits a linear range from 1pg/mL to 100ng/mL, a detection limit of 1pg/mL at a sensitivity of 2.02μA M(-1)at a regression coefficient of 0.99.

Publication types

  • Evaluation Study

MeSH terms

  • Antibodies, Immobilized / chemistry
  • Antibodies, Immobilized / immunology
  • Antibodies, Monoclonal / chemistry
  • Antibodies, Monoclonal / immunology
  • Biosensing Techniques / methods
  • Electrochemical Techniques / methods*
  • Electrodes
  • ErbB Receptors / analysis*
  • ErbB Receptors / immunology
  • Gold / chemistry
  • Humans
  • Immunoassay / methods*
  • Limit of Detection
  • Propionates / chemistry


  • Antibodies, Immobilized
  • Antibodies, Monoclonal
  • Propionates
  • Gold
  • ErbB Receptors