Chimeric flagellin as the self-adjuvanting antigen for the activation of immune response against Helicobacter pylori

Vaccine. 2012 Aug 31;30(40):5856-63. doi: 10.1016/j.vaccine.2012.07.011. Epub 2012 Jul 20.


Helicobacter pylori infection can cause gastritis, peptic ulcer and can lead to gastric cancer. Lengthy antibiotic therapy does not protect the host against reinfection. H. pylori evolved to evade the recognition of the immune response by modifying several of its components whose orthologous proteins from other bacteria activate the innate immune response. Flagella are essential for the H. pylori effective colonization of human duodenum and stomach. TLR5, a member of the Toll-like receptor family, recognizes flagellin of most bacteria, such as Escherichia coli, but does not recognize the flagellin FlaA of H. pylori. We restored the ability of FlaA for the recognition by TLR5 by engineering a chimeric flagellin, in which both terminal segments of H. pylori flagellin were replaced by the corresponding segments from TLR5-activating E. coli flagellin. Recombinant chimeric flagellin folded correctly and was able to activate TLR5. Significantly increased serum IgG and IgA antibody responses were determined in mice vaccinated with chimeric flagellin in comparison to mice vaccinated with a control protein (FlaA) or negative control. Antibody titers remained high even 8 months after the last immunization. Antibodies were able to bind native flagellin from H. pylori lysate. Vaccination with chimeric flagellin provided mice with significant protection against H. pylori. The approach of chimeric flagellin can therefore generate effective immunogens that enable activation of innate and adaptive immune response and can be used to construct efficient vaccines against H. pylori or other flagellated bacteria that evade TLR5 recognition.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptive Immunity
  • Animals
  • Antibodies, Bacterial / blood
  • Antigens, Bacterial / immunology*
  • Bacterial Vaccines / immunology*
  • Escherichia coli / immunology
  • Flagellin / immunology*
  • HEK293 Cells
  • Helicobacter Infections / immunology*
  • Helicobacter Infections / prevention & control
  • Helicobacter pylori / immunology*
  • Humans
  • Immunity, Innate
  • Immunodominant Epitopes / immunology
  • Immunoglobulin A / blood
  • Immunoglobulin G / blood
  • Mice
  • Mice, Inbred BALB C
  • Recombinant Fusion Proteins / immunology
  • Toll-Like Receptor 5 / immunology


  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Bacterial Vaccines
  • Immunodominant Epitopes
  • Immunoglobulin A
  • Immunoglobulin G
  • Recombinant Fusion Proteins
  • Toll-Like Receptor 5
  • Flagellin
  • flaA protein, bacteria