Topical effects of nebivolol on wounds in diabetic rats

Eur J Pharm Sci. 2012 Sep 29;47(2):451-5. doi: 10.1016/j.ejps.2012.06.017. Epub 2012 Jul 20.


Objective: Recently, it has reported that nebivolol might be useful in the treatment of diabetes mellitus foot ulcers. The aim of this study was to examine treatment of the wounds in streptozotocin-induced diabetic rats with topical nebivolol.

Methods: Two 15 × 15 mm-sized wounds were created in 56 streptozotocin-induced rats. A total of 56 diabetic wounds were studied in eight groups (n=7). No treatment was administered to the first and second groups. The third and fourth groups consisted of diabetic rats that were administered 1:1 mixture of lanolin and vaseline for 7 and 14 days, respectively. Five percent nebivolol plus 1:1 mixture of lanolin and vaseline was administered to rats in the fifth and sixth groups for 7 and 14 days, respectively, and 10% nebivolol plus 1:1 mixture of lanolin and vaseline was administered to rats in the seventh and eighth groups for 7 and 14 days, respectively. On days 7 and 14, wound healing was observed, and the percent of wound healing was determined by measuring its size and histopathologic examination. The ratio was calculated by the formula, healing ratio (%)=100 ×(1-wound area/initial wound area). Statistical analysis was performed by ANOVA with Tukey's HSD test and Mann-Whitney U test, using SPSS 15.0 software.

Results: On days 7 and 14, rates of wound healing in the fifth, sixth, seventh, and eighth groups were 57.42%, 89.16%, 60.80%, and 91.80%, respectively. Multiple comparison showed that rates of wound healing were significantly higher in rats administered 5% and 10% nebivolol than those in rats administered a mixture of lanolin and vaseline and in the untreated group (P<0.05).

Conclusion: Topical nebivolol therapy may be useful for wound healing in diabetic rats. Further studies are needed to support these data.

MeSH terms

  • Administration, Cutaneous
  • Adrenergic beta-1 Receptor Antagonists / administration & dosage
  • Animals
  • Benzopyrans / administration & dosage*
  • Diabetes Mellitus, Experimental / drug therapy*
  • Diabetes Mellitus, Experimental / pathology
  • Diabetic Foot / drug therapy*
  • Diabetic Foot / pathology
  • Ethanolamines / administration & dosage*
  • Male
  • Nebivolol
  • Rats
  • Rats, Wistar
  • Vasodilator Agents / administration & dosage
  • Wound Healing / drug effects*


  • Adrenergic beta-1 Receptor Antagonists
  • Benzopyrans
  • Ethanolamines
  • Vasodilator Agents
  • Nebivolol