Hypoxia triggers meiotic fate acquisition in maize

Science. 2012 Jul 20;337(6092):345-8. doi: 10.1126/science.1220080.

Abstract

Evidence from confocal microscopic reconstruction of maize anther development in fertile, mac1 (excess germ cells), and msca1 (no germ cells) flowers indicates that the male germ line is multiclonal and uses the MAC1 protein to organize the somatic niche. Furthermore, we identified redox status as a determinant of germ cell fate, defining a mechanism distinct from the animal germ cell lineage. Decreasing oxygen or H(2)O(2) increases germ cell numbers, stimulates superficial germ cell formation, and rescues germinal differentiation in msca1 flowers. Conversely, oxidizing environments inhibit germ cell specification and cause ectopic differentiation in deeper tissues. We propose that hypoxia, arising naturally within growing anther tissue, acts as a positional cue to set germ cell fate.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Anaerobiosis
  • Cell Hypoxia
  • Cells, Cultured
  • Germ Cells / metabolism
  • Meiosis*
  • Oxygen / metabolism*
  • Oxygen / pharmacology
  • Plant Epidermis / cytology
  • Plant Epidermis / drug effects
  • Plant Epidermis / metabolism
  • Water / metabolism
  • Water / pharmacology
  • Zea mays / cytology*
  • Zea mays / drug effects
  • Zea mays / genetics*

Substances

  • Water
  • Oxygen

Associated data

  • GEO/GSE39101