Optimization of hydrolysis conditions for the production of angiotensin-I converting enzyme-inhibitory peptides and isolation of a novel peptide from lizard fish (Saurida elongata) muscle protein hydrolysate

Mar Drugs. 2012 May;10(5):1066-80. doi: 10.3390/md10051066. Epub 2012 May 18.

Abstract

Lizard fish (Saurida elongata) muscle protein was hydrolyzed using neutral protease to produce protein hydrolysate (LFPH), and the hydrolysis conditions were investigated using response-surface methodology. The optimum conditions for producing peptides with the highest angiotensin-I converting enzyme (ACE)-inhibitory activity were the following: enzyme-to-substrate ratio of 10,000 U/g, temperature of 48 °C, pH 7.0, and hydrolysis time of 2 h. Under these conditions, the ACE-inhibitory activity of LFPH and the degree of hydrolysis were 84% and 24%, respectively. A novel ACE-inhibitory peptide was isolated from LFPH using ultrafiltration, Sephadex G-15, and high-performance liquid chromatography. The amino acid sequence of the ACE-inhibitory peptide was identified as Ser-Pro-Arg-Cys-Arg (SPRCR), and its IC₅₀ was 41 ± 1 µM.

Keywords: ACE-inhibitory peptide; enzymatic hydrolysis; isolation; lizard fish; response surface methodology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Angiotensin-Converting Enzyme Inhibitors / chemistry
  • Angiotensin-Converting Enzyme Inhibitors / isolation & purification*
  • Angiotensin-Converting Enzyme Inhibitors / metabolism*
  • Animals
  • Chromatography, High Pressure Liquid / methods
  • Fishes / metabolism
  • Hydrogen-Ion Concentration
  • Hydrolysis
  • Muscle Proteins / chemistry
  • Muscle Proteins / isolation & purification*
  • Muscle Proteins / metabolism
  • Peptide Hydrolases / chemistry
  • Peptide Hydrolases / metabolism
  • Peptides / chemistry
  • Peptides / isolation & purification*
  • Peptides / metabolism
  • Protein Hydrolysates / chemistry
  • Protein Hydrolysates / isolation & purification*
  • Protein Hydrolysates / metabolism
  • Temperature

Substances

  • Angiotensin-Converting Enzyme Inhibitors
  • Muscle Proteins
  • Peptides
  • Protein Hydrolysates
  • Peptide Hydrolases