Abstract
A photoluminescence probe ARC-1185, possessing both high affinity towards basophilic protein kinases (PKs) and microsecond-scale luminescence lifetime when associated with a kinase, was used for the mapping of ARC-1185-PK complexes in living cells with time-gated luminescence microscopy.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Basophils / enzymology
-
Dogs
-
Fluorescent Dyes / chemistry*
-
Madin Darby Canine Kidney Cells
-
Microscopy, Fluorescence*
-
Oligopeptides / chemistry*
-
Protein Kinases / chemistry
-
Protein Kinases / metabolism*
-
Time Factors
-
Ultraviolet Rays
Substances
-
ARC-1185
-
Fluorescent Dyes
-
Oligopeptides
-
Protein Kinases