Time-gated luminescence microscopy with responsive nonmetal probes for mapping activity of protein kinases in living cells

Chem Commun (Camb). 2012 Sep 4;48(68):8595-7. doi: 10.1039/c2cc33565d. Epub 2012 Jul 20.

Abstract

A photoluminescence probe ARC-1185, possessing both high affinity towards basophilic protein kinases (PKs) and microsecond-scale luminescence lifetime when associated with a kinase, was used for the mapping of ARC-1185-PK complexes in living cells with time-gated luminescence microscopy.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Basophils / enzymology
  • Dogs
  • Fluorescent Dyes / chemistry*
  • Madin Darby Canine Kidney Cells
  • Microscopy, Fluorescence*
  • Oligopeptides / chemistry*
  • Protein Kinases / chemistry
  • Protein Kinases / metabolism*
  • Time Factors
  • Ultraviolet Rays

Substances

  • ARC-1185
  • Fluorescent Dyes
  • Oligopeptides
  • Protein Kinases