Plasma cytokines as potential response indicators to dietary freeze-dried black raspberries in colorectal cancer patients

Nutr Cancer. 2012 Aug;64(6):820-5. doi: 10.1080/01635581.2012.697597. Epub 2012 Jul 24.

Abstract

Oral consumption of freeze-dried black raspberries attenuated neoplastic changes in colorectal tissue markers of apoptosis, cell proliferation, and angiogenesis in colorectal cancer (CRC) patients. To determine whether plasma concentrations of interleukin (IL)-1β, IL-2, IL-6, IL-8, IL-10, IL-12p70, granulocyte macrophage colony stimulating factor (GM-CSF), interferon-γ, and tumor necrosis factor-α (TNF-α) were associated with berry treatment and changes in colorectal tissue markers of apoptosis, cell proliferation, and angiogenesis, plasma and biopsy samples of adenocarcinoma and adjacent normal-appearing colorectal tissue were collected before and during berry treatment from 24 CRC patients who had not received prior therapy and drank a slurry of black raspberry powder (20 g in 100 ml drinking water) 3 times a day for 1 to 9 wk. Plasma concentrations of GM-CSF (+0.12 ± 0.04 pg/mL; P = 0.01) and IL-8 (-1.61 ± 0.71 pg/mL; P = 0.04) changed in patients receiving berries for more than 10 days. These changes were correlated with beneficial changes in markers of proliferation (r(ΔGM-CSF, ΔKi67 carcinoma - normal) = -0.51) and apoptosis (r(ΔIL-8, ΔTUNEL carcinoma - normal) = -0.52) observed in colorectal tissue taken within the same week. Plasma concentrations of GM-CSF and IL-8 may serve as noninvasive indicators to monitor tissue response to berry-based interventions for CRC.

Publication types

  • Clinical Trial
  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adenocarcinoma / blood
  • Adenocarcinoma / diet therapy
  • Adenocarcinoma / drug therapy*
  • Adenocarcinoma / pathology
  • Administration, Oral
  • Adult
  • Aged
  • Apoptosis
  • Biomarkers / blood
  • Colorectal Neoplasms / blood
  • Colorectal Neoplasms / diet therapy
  • Colorectal Neoplasms / drug therapy*
  • Colorectal Neoplasms / pathology
  • Cytokines / blood*
  • Female
  • Food Preservation
  • Freeze Drying
  • Fruit*
  • Granulocyte-Macrophage Colony-Stimulating Factor / blood
  • Humans
  • Interferon-gamma / blood
  • Interleukin-8 / blood
  • Interleukins / blood
  • Male
  • Middle Aged
  • Phytotherapy / methods
  • Predictive Value of Tests
  • Rosaceae*
  • Tumor Necrosis Factor-alpha / blood

Substances

  • Biomarkers
  • Cytokines
  • Interleukin-8
  • Interleukins
  • Tumor Necrosis Factor-alpha
  • Interferon-gamma
  • Granulocyte-Macrophage Colony-Stimulating Factor