Schizandrin, an antioxidant lignan from Schisandra chinensis, ameliorates Aβ1-42-induced memory impairment in mice

Oxid Med Cell Longev. 2012;2012:721721. doi: 10.1155/2012/721721. Epub 2012 Jul 4.

Abstract

In the present study, we examined the effect of schisandrin (SCH) of Schisandra chinensis on the amyloid-beta(1-42)- (Aβ(1-42)-) induced memory impairment in mice and elucidated the possible antioxidative mechanism. Mice were intracerebroventricular (i.c.v.) injected with the aggregated Aβ(1-42) and then treated with SCH (4, 12, and 36 mg/kg body weight) or donepezil (DPZ), a reference drug (0.65 mg/kg) by intragastric infusion for 14 days. Noncognitive disturbances and cognitive performance were evaluated by locomotor activity test, Y-maze test, and water maze test. Antioxidative enzyme activities including superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) and levels of malondialdehyde (MDA), glutathione (GSH), and oxidized glutathione (GSSG) within the cerebral cortex and hippocampus of mice were measured to elucidate the mechanism. Our results showed that SCH significantly improved Aβ(1-42)-induced short-term and spatial reference memory impairments in Y-maze test and water maze test. Furthermore, in the cerebral cortex and hippocampus of mice, SOD and GSH-px activities, GSH level, and GSH/GSSG ratio were increased, and levels of MDA and GSSG were decreased by the treatment of SCH. These results suggest that SCH is a potential cognitive enhancer against Alzheimer's disease through antioxidative action.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amyloid beta-Peptides / toxicity*
  • Animals
  • Antioxidants / chemistry
  • Antioxidants / metabolism
  • Antioxidants / pharmacology
  • Antioxidants / therapeutic use*
  • Biological Assay
  • Cerebral Cortex / drug effects
  • Cerebral Cortex / enzymology
  • Cerebral Cortex / pathology
  • Cerebral Cortex / physiopathology
  • Cyclooctanes / chemistry
  • Cyclooctanes / pharmacology
  • Cyclooctanes / therapeutic use*
  • Hippocampus / drug effects
  • Hippocampus / enzymology
  • Hippocampus / pathology
  • Hippocampus / physiopathology
  • Lignans / chemistry
  • Lignans / pharmacology
  • Lignans / therapeutic use*
  • Male
  • Maze Learning / drug effects
  • Memory Disorders / drug therapy*
  • Memory Disorders / physiopathology
  • Mice
  • Motor Activity / drug effects
  • Peptide Fragments / toxicity*
  • Polycyclic Compounds / chemistry
  • Polycyclic Compounds / pharmacology
  • Polycyclic Compounds / therapeutic use*
  • Reaction Time / drug effects
  • Schisandra / chemistry*

Substances

  • Amyloid beta-Peptides
  • Antioxidants
  • Cyclooctanes
  • Lignans
  • Peptide Fragments
  • Polycyclic Compounds
  • amyloid beta-protein (1-42)
  • schizandrin