Go hybrid: EM, crystallography, and beyond

Curr Opin Struct Biol. 2012 Oct;22(5):627-35. doi: 10.1016/j.sbi.2012.07.006. Epub 2012 Jul 24.


A mechanistic understanding of the molecular transactions that govern cellular function requires knowledge of the dynamic organization of the macromolecular machines involved in these processes. Structural biologists employ a variety of biophysical methods to study large macromolecular complexes, but no single technique is likely to provide a complete description of the structure-function relationship of all the constituent components. Since structural studies generally only provide snapshots of these dynamic machines as they accomplish their molecular functions, combining data from many methodologies is crucial to our understanding of molecular function.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Crystallography, X-Ray / methods*
  • Humans
  • Macromolecular Substances / chemistry
  • Microscopy, Electron / methods*


  • Macromolecular Substances