Introduction: Metabolic syndrome (MetS) is a multifactorial disorder in which dyslipidemia plays an important role. Fatty acid-binding protein 2 (FABP 2) is responsible for transport of free fatty acids in the intestinal endothelium cells. FABP2-genetic variants might affect plasma lipid concentrations and intracellular lipid transport. The aim of this study was to investigate the association between FABP2 Ala54Thr genetic polymorphism and metabolic syndrome and some biochemical and anthropological parameters in elderly subjects.
Materials and methods: This cross-sectional study included 140 men and 176 women older than 70 years. Fasting serum concentration of glucose, lipid parameters, total proteins and C-reactive protein were determined by standardized methods. Presence (MetS(+)) or absence (MetS(-)) of MetS was determined according to criteria of the International Diabetes Federation. FABP2 genetic polymorphism Ala54Thr (rs1799883) was genotyped with PCR-RFPL.
Results: The genotype frequencies for Ala/Ala, Ala/Thr and Thr/Thr genotype were 60, 36 and 6 in MetS(-), and 131, 70 and 13 in MetS(+), respectively, without statistical significance (P = 0.567). Ala/Ala genotype was a subgroup of non-carriers, while Ala/Thr and Thr/Thr genotypes were Thr54-carriers. Median triglyceride concentration was significantly lower in carriers then in non-carriers for whole MetS(+) group (P = 0.050); there were no significant difference between men with MetS (P = 0.144), but there was a difference between women with MetS (P = 0.020). T-test showed that mean HDL cholesterol concentrations in MetS(+) group for Thr54-carriers was significantly higher in whole group (P = 0.001), and for both genders (men P = 0.039; women P = 0.004) as compared to non-carriers.
Conclusions: FABP2 genetic polymorphism is associated with lower triglyceride and higher HDL-cholesterol concentrations in elderly subjects with MetS. This genetic variation might be a useful marker for understanding dyslipidemia in MetS.
Uvod:: Metabolički sindrom (MetS) je multifaktorijelni poremećaj kod kojeg dislipidemija ima ključnu ulogu. Protein-nosač masnih kiselina 2 (FABP 2) je odgovoran za prijenos slobodnih masnih kiselina u stanicama crijevnog endotela. Genske varijante mogu utjecati na promjene koncentracija lipida u plazmi kao i na unutarstanični prijenos lipida. Cilj ovog istraživanja je ispitati povezanost polimorfizma Ala54Thr gena FABP2 i metaboličkog sindroma, antropometrijskih i biokemijskih parametara u starijih osoba.
Materijali i metode:: U istraživanje je uključeno 140 muškaraca i 176 žena iznad 70 godina starosti. Koncentracije glukoze, lipidnih parametara, ukupnih proteina i C-reaktivnog proteina određene su standardnim metodama u uzorcima seruma uzetim natašte. Prisutnost (MetS(+)) ili odsutnost (MetS(−)) MetS su određene na temelju kriterija Međunarodne federacije za šećernu bolest. Genski polimorfizam FABP2, Ala 54Thr (rs1799883) je genotipiziran metodom PCR-RFLP.
Rezultati:: Učestalost genotipova Ala/Ala, Ala/Thr i Thr/Thr bila je redom 60, 36 i 6 kod MetS(−) ispitanika, te 131, 70 i 13 kod MetS(+) ispitanika, bez statistički značajne razlike (P = 0,567). Genotip Ala/Ala predstavlja skupinu nenositelja dok su genotipovi Ala/Thr i Thr/Thr nositelji Thr54 alela. Median koncentracije triglicerida je bio značajno niži kod Thr-nositelja u cjelovitoj MetS(+) skupini (P = 0,050), bez značajne razlike kod muškaraca (P = 0,144), ali sa značajnom razlikom kod žena s MetS (P = 0,020). T-test je pokazao da je srednja vrijednost koncentracije HDL-kolesterola Thr54-nositelja značajno viša u cjelovitoj skupini s MetS (P = 0,001) i to kod oba spola (muškarci P = 0,039; žene P = 0,004).
Zaključci:: Genski polimorfizam FABP2 je povezan s nižom koncentracijom triglicerida te višom koncentracijom HDL-kolesterola kod starije populacije s MetS. Ova genska varijanta može biti koristan biljeg za razumijevanje dislipidemije kod MetS.