Platelet-derived growth factor oncoprotein detection using three-dimensional carbon microarrays

Biosens Bioelectron. 2013 Jan 15;39(1):118-23. doi: 10.1016/j.bios.2012.06.055. Epub 2012 Jul 20.


The potential of aptamers as ligand binding molecule has opened new avenues in the development of biosensors for cancer oncoproteins. In this paper, a label-free detection strategy using signaling aptamer/protein binding complex for platelet-derived growth factor (PDGF-BB) oncoprotein detection is reported. The detection mechanism is based on the release of fluorophore (TOTO intercalating dye) from the target binding aptamer's stem structure when it captures PDGF. Amino-terminated three-dimensional carbon microarrays fabricated by pyrolyzing patterned photoresist were used as a detection platform. The sensor showed near linear relationship between the relative fluorescence difference and protein concentration even in the sub-nanomolar range with an excellent detection limit of 5pmol. This detection strategy is promising in a wide range of applications in the detection of cancer biomarkers and other proteins.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Aptamers, Nucleotide / chemistry*
  • Becaplermin
  • Biosensing Techniques / instrumentation
  • Carbon / chemistry*
  • Equipment Design
  • Humans
  • Limit of Detection
  • Protein Array Analysis / instrumentation*
  • Proto-Oncogene Proteins c-sis / analysis*


  • Aptamers, Nucleotide
  • Proto-Oncogene Proteins c-sis
  • Becaplermin
  • Carbon