Three structural representatives of the PF06855 protein domain family from Staphyloccocus aureus and Bacillus subtilis have SAM domain-like folds and different functions

J Struct Funct Genomics. 2012 Sep;13(3):163-70. doi: 10.1007/s10969-012-9134-6. Epub 2012 Jul 29.


Protein domain family PF06855 (DUF1250) is a family of small domains of unknown function found only in bacteria, and mostly in the order Bacillales and Lactobacillales. Here we describe the solution NMR or X-ray crystal structures of three representatives of this domain family, MW0776 and MW1311 from Staphyloccocus aureus and yozE from Bacillus subtilis. All three proteins adopt a four-helix motif similar to sterile alpha motif (SAM) domains. Phylogenetic analysis classifies MW1311 and yozE as functionally equivalent proteins of the UPF0346 family of unknown function, but excludes MW0776, which likely has a different biological function. Our structural characterization of the three domains supports this separation of function. The structures of MW0776, MW1311, and yozE constitute the first structural representatives from this protein domain family.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Amino Acid Motifs
  • Amino Acid Sequence
  • Bacillus subtilis / chemistry*
  • Bacillus subtilis / classification
  • Bacillus subtilis / genetics
  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / classification
  • Bacterial Proteins / genetics
  • Cloning, Molecular
  • Crystallography, X-Ray
  • Genes, Bacterial
  • Magnetic Resonance Spectroscopy / methods
  • Molecular Sequence Data
  • Phylogeny
  • Protein Folding*
  • Protein Structure, Secondary
  • Protein Structure, Tertiary
  • Sequence Alignment
  • Species Specificity
  • Staphylococcus aureus / chemistry*
  • Staphylococcus aureus / classification
  • Staphylococcus aureus / genetics
  • Structure-Activity Relationship


  • Bacterial Proteins