Oceanobacillus indicireducens sp. nov., a facultative alkaliphile that reduces an indigo dye

Int J Syst Evol Microbiol. 2013 Apr;63(Pt 4):1437-1442. doi: 10.1099/ijs.0.034579-0. Epub 2012 Jul 27.

Abstract

An indigo-reducing facultatively alkaliphilic and halophilic strain, designated strain A21(T), was isolated from a fermented Polygonum indigo (Polygonum tinctorium Lour.) liquor sample aged for 4 days prepared in a laboratory. 16S rRNA gene sequence phylogeny suggested that strain A21(T) was a member of the genus Oceanobacillus with the closest relative being the type strain of Oceanobacillus chironomi (similarity: 96.0 %). The cells of the isolate stained Gram-positive and were facultatively anaerobic straight rods that were motile by peritrichous flagella. The strain grew between 18 and 48 °C with optimum growth at 39 °C. It grew in the pH range of 7-12. It hydrolysed casein, gelatin and Tween 20 but not Tweens 40, 60 and 80, starch or DNA. No isoprenoid quinone was detected and the DNA G+C content was 39.7 mol%. The whole-cell fatty acid profile mainly consisted of iso-C15 : 0, anteiso-C15 : 0 and C16 : 0. DNA-DNA hybridization experiments with O. chironomi revealed 13 % relatedness. Owing to the differences in phenotypic and chemotaxonomic characteristics, and phylogenetic analyses based on 16S rRNA gene sequences and DNA-DNA relatedness data from reported Oceanobacillus species, the isolate merits classification as a representative of a novel species, for which the name Oceanobacillus indicireducens sp. nov. is proposed. The type strain is A21(T) ( = JCM 17251(T) = NCIMB 14685(T)). The description of the genus Oceanobacillus is also emended.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillaceae / classification*
  • Bacillaceae / genetics
  • Bacillaceae / isolation & purification
  • Bacillaceae / metabolism
  • Bacterial Typing Techniques
  • Base Composition
  • Coloring Agents / metabolism*
  • DNA, Bacterial / genetics
  • Fatty Acids / analysis
  • Fermentation
  • Indigo Carmine
  • Indoles / metabolism*
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Phylogeny*
  • Polygonum
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA

Substances

  • Coloring Agents
  • DNA, Bacterial
  • Fatty Acids
  • Indoles
  • RNA, Ribosomal, 16S
  • Indigo Carmine

Associated data

  • GENBANK/AB623011