Removal of Artifactual Bands Associated With the Presence of 2-mercaptoethanol in Two-Dimensional Polyacrylamide Gel Electrophoresis

Anal Biochem. 1990 Oct;190(1):57-9. doi: 10.1016/0003-2697(90)90132-s.

Abstract

A method for eliminating artifactual bands due to the presence of 2-mercaptoethanol in two-dimensional gels is described. The method is based on a modification of the procedure of application of the first dimension gel to the SDS slab gel. 2-Mercaptoethanol is removed during equilibration and replaced by iodoacetamide. The use of iodoacetamide improves the recovery of proteins and results in a better detection of them.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Membrane / chemistry
  • Electrophoresis, Gel, Two-Dimensional / methods
  • Membrane Proteins / analysis
  • Mercaptoethanol / analysis*
  • Mercaptoethanol / chemistry
  • Oocytes / ultrastructure
  • Rana ridibunda
  • Silver
  • Sodium Dodecyl Sulfate

Substances

  • Membrane Proteins
  • Sodium Dodecyl Sulfate
  • Silver
  • Mercaptoethanol