Hydrogen peroxide induced activation of gene expression in mammalian cells using boronate estrone derivatives

Jeane M Govan; Andrew L McIver; Chad Riggsbee; Alexander Deiters

doi:10.1002/anie.201203222

Hydrogen peroxide induced activation of gene expression in mammalian cells using boronate estrone derivatives

Angew Chem Int Ed Engl. 2012 Sep 3;51(36):9066-70. doi: 10.1002/anie.201203222. Epub 2012 Jul 31.

Authors

Jeane M Govan¹, Andrew L McIver, Chad Riggsbee, Alexander Deiters

Affiliation

¹ Department of Chemistry, North Carolina State University, Raleigh, NC 27695-8204, USA.

PMID: 22855386
DOI: 10.1002/anie.201203222

Abstract

Keeping the boron out of the ER: A genetic switch was engineered that activates gene expression in the presence of H(2)O(2). The use of a boronate group on an estrone molecule allows for activation of gene expression through binding of the estrogen receptor only when the boron group is oxidized by H(2)O(2). This sensor is highly sensitive and specific for H(2)O(2).