Nasal Carriage as a Source of Agr-Defective Staphylococcus Aureus Bacteremia

J Infect Dis. 2012 Oct;206(8):1168-77. doi: 10.1093/infdis/jis483. Epub 2012 Aug 2.


Inactivating mutations in the Staphylococcus aureus virulence regulator agr are associated with worse outcomes in bacteremic patients. However, whether agr dysfunction is primarily a cause or a consequence of early bacteremia is unknown. Analysis of 158 paired S. aureus clones from blood and nasal carriage sites in individual patients revealed that recovery of an agr-defective mutant from blood was usually predicted by the agr functionality of carriage isolates. Many agr-positive blood isolates produced low levels of hemolytic toxins, but levels were similar to those of colonizing strains within patients, suggesting that introduction into the blood did not select for mutations with minor functional effects. Evidently, the transition from commensalism to opportunism in S. aureus does not require full virulence in hospitalized patients. Furthermore, agr-defective mutants were found in uninfected nasal carriers in the same proportion as in carriers who develop bacteremia, suggesting low correlation between virulence and infectivity.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteremia / microbiology*
  • Bacterial Proteins / classification
  • Bacterial Proteins / genetics
  • Carrier State / microbiology*
  • Genotype
  • Humans
  • Methicillin-Resistant Staphylococcus aureus / genetics*
  • Methicillin-Resistant Staphylococcus aureus / isolation & purification
  • Molecular Typing
  • Nasal Mucosa / microbiology
  • Sequence Analysis, DNA
  • Staphylococcal Infections / microbiology*
  • Trans-Activators / classification
  • Trans-Activators / deficiency*
  • Trans-Activators / genetics
  • Virulence


  • Agr protein, Staphylococcus aureus
  • Bacterial Proteins
  • Trans-Activators