Involvement of AMPA receptors in CSD-induced impairment of LTP in the hippocampus

Headache. 2012 Nov-Dec;52(10):1535-45. doi: 10.1111/j.1526-4610.2012.02229.x. Epub 2012 Aug 3.

Abstract

Objective: To investigate the alteration of hippocampal long-term plasticity and basal synaptic transmission induced by repetitive cortical spreading depressions (CSDs).

Background: There is a relationship between migraine aura and amnesia attack. CSD, a state underlying migraine attacks, may be responsible for hippocampus-related symptoms. However, the precise role of CSD on hippocampal activity has not been investigated.

Methods: Male Wistar rats were divided into CSD and control groups. Repetitive CSDs were induced in vivo by topical application of solid KCl. Forty-five minutes later, the ipsilateral hippocampus was removed, and hippocampal slices were prepared for a series of electrophysiological studies.

Results: Repetitive CSDs led to a decrease in the magnitude of long-term potentiation in the hippocampus. CSD also reduced hippocampal synaptic efficacy, as shown by a reduction in post-synaptic α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor responses. In contrast, the post-synaptic N-methyl-d-aspartate receptor responses remained unchanged. In addition, there were no changes in paired-pulse profiles between the groups, indicating that CSD did not induce any presynaptic alterations.

Conclusion: These findings suggest that a reduction of post-synaptic α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor responses is the mechanism responsible for impaired hippocampal long-term potentiation induced by CSD.

Keywords: cortical spreading depression; hippocampus; long-term potentiation; α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cortical Spreading Depression / physiology*
  • Hippocampus / physiology*
  • Long-Term Potentiation / physiology*
  • Male
  • Organ Culture Techniques
  • Rats, Wistar
  • Receptors, AMPA / physiology*

Substances

  • Receptors, AMPA