Effects of adding a concentrated pomegranate-residue extract to the ration of lactating cows on in vivo digestibility and profile of rumen bacterial population

J Dairy Sci. 2012 Oct;95(10):5996-6005. doi: 10.3168/jds.2012-5537. Epub 2012 Aug 3.


This study characterizes the effects of concentrated pomegranate-peel extract (CPE) addition to the TMR at levels of 1, 2, or 4% on voluntary intake, in vivo digestibility, milk yield and composition, and profile of rumen bacterial and archaeal populations in lactating Holstein cows. Supplementation of CPE significantly affected the abundance of methanogenic archaea and specific ruminal bacterial species related to cellulolytic activities and soluble sugar and lactic acid fermentation, as revealed by real-time PCR quantification. Furthermore, CPE supplementation had a significant dose-dependent effect on the whole ruminal bacterial community, as determined by automated ribosomal intergenic spacer analysis. These changes were accompanied by a significant increase in digestibility of dry matter, crude protein, and neutral detergent fiber, as well as milk and energy-corrected milk yields in cows fed the 4% CPE supplement. These results suggest that CPE supplementation significantly affects the rumen bacterial communities, which in turn may be related to a beneficial effect on dairy cow performance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animal Nutritional Physiological Phenomena / drug effects
  • Animal Nutritional Physiological Phenomena / physiology
  • Animals
  • Cattle / metabolism
  • Cattle / physiology*
  • DNA, Bacterial / genetics
  • DNA, Ribosomal Spacer / genetics
  • Diet / veterinary
  • Dietary Supplements
  • Digestion / drug effects*
  • Digestion / physiology
  • Dose-Response Relationship, Drug
  • Female
  • Fruit
  • Hydrogen-Ion Concentration
  • Lactation / drug effects*
  • Lactation / physiology
  • Lythraceae*
  • Milk / chemistry
  • Plant Extracts / pharmacology*
  • Real-Time Polymerase Chain Reaction / veterinary
  • Rumen / drug effects*
  • Rumen / microbiology


  • DNA, Bacterial
  • DNA, Ribosomal Spacer
  • Plant Extracts